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Gel ltration beads covering a particular molecular mass range are selected and packed into a column (Table 2.3). Unlike other chromatography formats column geometry is important in this experiment. Gel ltration columns are usually long relative to their column diameter. However, the low tolerance of gel ltration resins to hydrostatic pressure places an upper limit on the volume of resin which may be used (i.e. the column cannot be too long). Conversely, the loading volume of sample is also limited (usually 1 2% of the total column volume) which means that the column volume cannot be too small either. Once packed, the void volume of the column is determined by passing a solution of blue dextran through it and measuring its elution volume. Standard proteins of known native Mr are then applied to the column to calibrate it. These may be applied singly or in pairs (provided the two proteins are well-separated on the column and can clearly be distinguished by some other technique such as spectroscopy or electrophoresis). In selecting standards it is important that they are comparable to the sample protein under investigation (i.e. that they have the same general shape characteristics).

Much of the preceding code deals with connection and resource management along with try-catch-finally blocks for exception handling, which hinder the actual code logic and obscure the task that the code is intended to accomplish. These tedious tasks of JDBC have led to the creation of publicly available JDBC frameworks or in-house JDBC abstractions. The Spring JDBC framework simplifies this JDBC integration using a template-based approach wherein the update operation can be done with just a few lines of code. We ll discuss this a bit later in the chapter. In the following Try It Out exercise you ll learn how to work with JDBC. Later in this chapter you ll see firsthand how Spring makes it easier to use JDBC.

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Figure B-17 shows a relatively simple data model for team sports. This design is based on a typical volleyball league. Players belong to a team and teams play in tournaments.

To create a freestanding label, you need to have a table open with at least a link and a label template. If you have more than one template defined, choose the one that you want to use in the drop-down list at the top of the Data View window. Follow these steps: 1. Select the record that you want to link to the freestanding label. 2. Choose Data View Link and Label Settings Create Freestanding Labels. 3. Choose Data View Link!

Since PDAs are meant to be mobile, they suffer from all of the vulnerabilities that laptops do. You need to protect them from theft, sniffing and password theft, data theft, data corruption, and to a lesser extent, from viruses. Theft is probably the most common threat, and the one you can most easily prevent with vigilance. To protect against theft we recommend that you turn on the password security feature on your PDA. Both the Palm and PocketPC have security utilities that let you set a password in order to get access to the data on the device. Figure 18-9 shows this utility from a Palm V device. A password will protect your data from people with average computer skills; they will be forced to reset the device in order to eliminate the password, thus sending the device back to its default state. However, a password alone won t help you get your PDA back to you. For that, you need to have a method of identifying the device as yours. The single best thing to do is to have your name, address, and phone number engraved on the device. Although some utilities allow you to set a screen that shows this information, software is software and can be defeated or lost when the device is reset.

(9 kcal/g), each of which contribute 40 45% of total basal requirements. Most of the remainder is provided by oxidation of amino acids derived from protein breakdown, yielding 4 kcal/g (see 7). Tissue synthesis and maintenance demand additional energy cost in growing infants, children, pregnant and lactating women, as do tissue breakdown or repair due to disease or trauma. These extra energy costs may be re ected in some or all of the measurements discussed below, but are dif cult to quantify; estimates are usually derived from stoichiometric equations based on rates and composition of weight gain or loss, milk production, tissue synthesis and the effects of infection or trauma (FAO/WHO/UNU, 2004; Elia, 1992a, 1992b).

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