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HARVEY: I promise you that if you do this, if you take this first step, that I ll never threaten to stop helping you again. I promise you that you will make a huge step forward in your discipline. And that before you know it, we ll be working together again. ME: This is really hard for me. HARVEY: It s hard for me to see you in this spot, too. But I am not going to let you off the hook. So what s your answer ME: I ll do it. I will. I will go back to Mr. Johnson tomorrow and I ll ask for my job back. I can t believe that he would ever let me have it back, but I will ask. And if he says no, I ll find something else. HARVEY: Anything else ME: Right. I will deliver pizzas if I have to. But I will earn back the $5,000 that I lost. HARVEY: Agreed. Assuming you shape up this week, I want you to start on the next law of trading by yourself it s the law of testing, and I am going to leave you to figure out the details. If you can get that done, then we ll be seeing each other again. Now I have to go.

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Serum cholesterol exists as a mixture of fatty acid esters and free cholesterol. Quantitation of total cholesterol involves the initial conversion of the esters to free cholesterol, followed by the total conversion of free cholesterol to its oxidation product. This reaction is coupled to the familiar dye-peroxidase indicator reaction. The parameter A500 measurements using stock cholesterol solutions provide a calibration curve. A reagent blank solution is prepared using all components except cholesterol, and this value is subtracted from all measured A500 values, correcting for any background oxidation of the dye. Fixed-change assays are relatively uncommon, and are used for enzyme quantitation. These assays monitor the time required for the generation of a given concentration of product. The enzyme concentration is inversely related to the time required for this extent of reaction to occur, so that a linear plot of 1/t against [E] is used as a calibration curve. 3.5. INSTRUMENTAL METHODS A wide variety of instrumental methods have been used to quantitate enzymes and their substrates. The choice of method depends primarily on the physical properties of the species being measured, and this is generally the product of the enzymatic or indicator reaction. In this section, instrumental detection methods are broadly classi ed as optical, electrochemical or other , where other techniques include radiochemical and manometric methods. 3.5.1. Optical Detection 3.5.1.1. Absorbance. The detection of substrate depletion or product accumulation through the measurement of visible or UV light absorbance is based on the Beer Lambert law, which directly relates absorbance at a given wavelength to concentration: A ebC 3:19 where b is the path length of the sample through which light travels, and e is a constant for a particular molecular species at a given wavelength under xed conditions

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Recall that when a Java program is compiled, it compiles down to platform-independent Java byte code. As Figure 2.3 shows, Java byte code is verified before it can run. This verification scheme is meant to ensure that the byte code, which may or may not have been created by a Java compiler, plays by the rules. After all, byte code could well have been created by a "hostile compiler'' that assembled byte code meant to violate the rules of the Java VM; created directly with an editor like emacs; created almost directly with a Java byte code assembler like Jasmin; or compiled from another source language like C++, Scheme, or Ada into Java byte code. The important thing is not what the source code looked like or even what language it was written in, but what the byte code (the code that actually ends up running) does. In this sense, the Verifier makes mystery code a bit less mysterious.