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Figure 11.19 Cycle of peptide bond formation and translocation on the ribosome. (a) After the peptide bond is transferred ( g. 11.17), the ribosome and messenger RNA move over one codon. Now the transfer RNA with the peptide is in the P site, and the A site is again open. In this example, the next transfer RNA that moves into the A site carries glutamic acid. (b) Three-dimensional model of the translocation process minus the mRNA and amino acids. The tRNA in the A site is pale blue, the tRNA in the P site is green, and the tRNA in the E site is yellow, then brown when ready to leave. Going clockwise from a, in which the A and P sites are occupied: EF-G translocates the ribosome after peptide bond formation and then evacuates the A site. Ef-Tu brings a new charged tRNA to the A site while the E site is emptied. ([b] Courtesy of (b)

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88 Show that the DC voltage output of the full-wave recti er of Figure 842 is 2N vS max / 89 Compute the peak voltage output of the bridge recti er of Figure 844, assuming

810 Compute the actual DC load voltage and the percentage of the ripple reaching the load (relative to the initial 100-mV ripple) for the circuit of Example 810 if rZ = 1

Joachim Frank.)

sin ( + ) =

Signal-Processing Applications Among the numerous applications of diodes, there are a number of interesting signal-conditioning or signal-processing applications that are made possible by the nonlinear nature of the device We explore three such applications here: the diode limiter, or clipper; the diode clamp; and the peak detector Other applications are left for the homework problems

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In eukaryotes, however, almost all messenger RNAs contain the information for only one gene (monocistronic). Since most ribosomal recognition of eukaryotic genes depends on the 5 cap, and since each eukaryotic messenger RNA has only one cap, usually only one polypeptide can be translated for any given messenger RNA. Exceptions occur when the messenger RNAs contain internal ribosome entry sites. Although it is certainly not the rule, the translated peptide can be modified or cleaved into smaller functional peptides. For example, in mice, a single messenger RNA codes for a protein that is later cleaved into epidermal growth factor and at least seven other related peptides. In addition, the same sequence can, in some cases, give rise to alternative proteins through alterna-

The Diode Clipper (Limiter)

vS(t) + _

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In the last chapter, we brie y discussed the shape and composition of the ribosomal subunits.All of the protein and RNA components have been isolated.Assembly pathways are known.We know approximately where the messenger RNA,initiation factors,and EF-Tu are located on the 30S subunit during translation ( g. 11.24; cf. g. 11.18).We also know where peptidyl transferase activity and EF-G reside on the 50S subunit, which has a cleft leading into a tunnel that passes through the structure. At present, it seems that the nascent peptide passes through this

The diode clipper is a relatively simple diode circuit that is often employed to protect loads against excessive voltages The objective of the clipper circuit is to keep the load voltage within a range say, Vmax vL (t) Vmax so that the maximum allowable load voltage (or power) is never exceeded The circuit of Figure 858 accomplishes this goal The circuit of Figure 858 is most easily analyzed by rst considering just the branch containing D1 This corresponds to clipping only the positive peak

Applying the trigonometric identity,

voltages; the analysis of the negative voltage limiter is left as a drill exercise The circuit containing the D1 branch is sketched in Figure 859; note that we have exchanged the location of the D1 branch and that of the load branch for convenience Further, the circuit is reduced to Th venin equivalent form Having e reduced the circuit to a simpler form, we can now analyze its operation for two distinct cases: the ideal diode and the piecewise linear diode 1 Ideal diode model For the ideal diode case, we see immediately that D1 conducts if RL vS (t) Vmax rS + R L (825)

The McGraw Hill Companies, 2001

and that if this condition occurs, then (D1 being a short circuit) the load voltage, vL , becomes equal to Vmax The equivalent circuit for the on condition is shown in Figure 860

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