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HV ( = 2 10,000) =

10,000 4000 1+ 10,000 4000

Researchers in the mid-1950s assumed that the genetic code consisted of simple sequences of nucleotides specifying particular amino acids.They sought answers to questions such as: Is the code overlapping Are there nucleotides between code words (punctuation) How many letters make up a code word (codon) Logic, along with genetic experiments, supplied some of the answers, but only with the rapidly improving techniques of biochemistry did they eventually decode the genetic language.

= 0929 038

2 ( f R ) sin + + d = R R 1 2 d = R( f/R ) sin q d = f sin q (39) For typical conditions, q < 10 and sin q q d = fq The location of d is referenced by the distance Z measured from the tool centre to d on the plane of the cut as shown in the figure

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The experimental manipulation of mutant genes, primarily by Francis Crick and his colleagues, reinforced the triplet code concept. In these experiments, a chemical mutagen, the acridine dye pro avin, was used to cause inactivation of the rapid lysis (rIIB) gene of the bacteriophage T4. Pro avin inactivates the gene by either adding or deleting a nucleotide from the DNA (see chapter 12). The rII gene controls the plaque morphology of this bacteriophage growing on E. coli cells. Rapid-lysis mutants produce large plaques; the wild-type form of the gene, rII , results in normal plaque morphology. Figure 11.28 shows the consequences of adding or deleting a nucleotide. From the point of addition or deletion onward, a frameshift causes codons to be read in different groups of three. If a deletion is combined with an addition to produce a double-mutant gene, the frameshift occurs only in the region between the two mu-

1 Phase, degrees 08 Amplitude 06 04 02 0 101 10 2 103 104 105 106 Radian frequency (logarithmic scale) 107 80 60 40 20 0 101 10 2 103 104 105 106 Radian frequency (logarithmic scale) 107

6

Several lines of evidence seemed to indicate that the nature of the code was triplet (three bases in messenger RNA specifying one amino acid).If codons contained only one base, they would only be able to specify four amino acids since there are only four different bases in DNA (or messenger RNA).A couplet code would have 4 4 16 two-base words, or codons, which is still not enough to specify uniquely twenty different amino acids. A triplet

Comments: The effect of this high-pass lter is to preserve the amplitude of the input signal at frequencies substantially greater than 0 , while signals at frequencies below 0 would be strongly attenuated With 0 = 2 4,000 (ie, 4,000 Hz), this lter would pass only the treble range of the audio frequency spectrum

Band-Pass Filters Building on the principles developed in the preceding sections, we can also construct a circuit that acts as a band-pass lter, passing mainly those frequencies within a certain frequency range The analysis of a simple second-order band-pass lter (ie, a lter with two energy-storage elements) can be conducted by analogy with the preceding discussions of the low-pass and high-pass lters Consider the circuit shown in Figure 619, and the related frequency response function for the lter H (j ) = Noting that Vo (j ) = Vi (j ) R R + 1/j C + j L Vo (j ) Vi

The McGraw Hill Companies, 2001

using the following identity: cos (a + b) = cos a sin b sin a cos b Substituting for sin a and cos b from equation (34) and (35) results in the following evolvement of Z: Z =

RLC band-pass filter The circuit preserves frequencies within a band C L + + Vi _ R Vo _

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