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There s not just one DNA polymerase; there s a whole army DNA replication actually occurs in large complexes containing many proteins and sometimes many polymerases In eukaryotic cells we have to replicate both mitochondrial and nuclear DNA, and there are specific DNA polymerases for each In addition to DNA replication, you have to make new DNA when you repair Consequently, the function may be specialized for repair or replication There can also be specialization for making the leading or lagging strand Some of the activities of DNA polymerases from eukaryotes and prokaryotes are shown in the table on the next page DNA polymerases all synthesize new DNA using a template and make the new DNA in a 5 3 direction (new nucleotides are added to the 3 end) In addition to making DNA, some of the DNA polymerases can also hydrolyze it An exonuclease works only on the ends of the DNA (or RNA), and like everything else about DNA, exonuclease activity has a direction too The 3 5 exonuclease activity removes nucleotides from the 3 end (by hydrolyzing the phosophodiester bond) Since the chain grows in the 5 3 direction, polymerases that have a 3 5 exonuclease activity can look back over their work and remove what they just put in if it was wrong This is the only direction that proofreading will work The 5 3 exonuclease activity looks forward in the same direction as the new chain is growing Therefore, it can only remove things that it finds in front of it (such as RNA primers on the lagging strand)

FIGURE 2-3

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This section considers the system performance in simulation The simulation analysis is useful as both the actual and estimated states are known Therefore, the estimation error can be directly observed Figure 124 shows the vehicle position as a function of time in the tangent plane (dashed line), the estimated vehicle position (solid line), and the LBL computed positions (x s) The sharp corners along the trajectory are achieved by commanding the forward speed u to zero at each corner while the yaw angle changes by 90 Detailed analysis of data not included herein shows the following After approximately one minute of operation, the north and east position error standard deviations as predicted by the P matrix remain less that 015 m while the depth error standard deviation remains less that 001 m The peak errors in the position errors, as computed by subtracting the estimated position from the true position, are on the order of [02, 04, 001] m Further analysis of the simulation data yields the performance indicators shown in Table 123 Figure 125 shows the accelerometer bias ba (solid line) and its esti mate ba (dots) during the rst 20s of the simulation Also shown are the , 2 , and 3 curves, where represents the square root of the

Before leaving this discussion of MVRC, you should understand that Oracle has leveraged the internal processes and structures used to implement MVRC to add a set of flashback features to the database As the name implies, flashback is the ability to deliver a view of the database at an earlier point in time A number of flashback features are available in Oracle 11 g:

Last Change N/A N/A N/A N/A 2450 2120 1780 1540 1428 1155 925 785 575 435 300 186 114 064 033 017 010 000 000 000 000 000 000 000 000 111 050 035 115 080 065 045 026 022 014 007 002 000

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Flashback query allows users to run queries as if they had taken place in the past with the use of a couple of keywords in a standard SQL statement Flashback database gives administrators the ability to roll back an entire database to an earlier point in time Flashback drop gives you the ability to remove the effects of one of those unfortunate oops moments, when you mistakenly drop a database object

Figure 125: Accelerometer bias plots, ba Actual bias (solid); estimated bias (dotted); and , 2 , and 3 plots appropriate diagonal element of the P matrix At the end of the simulation (t = 265s), the vector of accelerometer bias standard deviations is [0002, 0002, 0001] m/s2 The standard deviation curves in Figure 125 are a covariance analysis demonstrating that the observability conclusions of Section 127 are valid Figure 126 shows the gyro bias bg (solid line) and its estimate bg (dots) during the rst 20s of the simulation Also shown are the , 2 , and 3 curves, where represents the square root of the appropriate diagonal element of the P matrix At the end of the simulation (t = 265s), the vector of gyro bias standard deviations is [07, 07, 15] 10 3 deg/s The navigation system is operational on an AUV being used in research related to ship hull inspection For the in-water data, the actual vehicle state is not known; therefore, the main data useful for analyzing the performance are the measurement residuals In-water data is presented and analyzed in [97, 98]

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