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tants. If this region is small enough or does not contain coding for vital amino acids, the function of the gene may be restored. Two deletions or two insertions combined will not restore the reading frame. However, Crick and his colleagues found that the combination of three additions or three deletions did restore gene function.This nding led to the conclusion that the genetic code was triplet,beNormal (CAG repeat) C A G C A G C A G C A G C A G First A deleted (AGC repeat) * C G C A G C A G C A G C A G C mRNA

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j CR = Vi (j ) 1 + j CR + (j )2 LC we may write the frequency response of the lter as Vo j CR (j ) = Vi 1 + j CR + (j )2 LC Equation 629 can often be factored into the following form: Vo j A (j ) = Vi (j / 1 + 1)(j / 2 + 1)

(628)

cause a triplet code would be put back into the reading frame by three additions or three deletions ( g. 11.29).

(629)

(40)

(630)

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Questions still remained: was the code overlapping Did it have punctuation Several logical arguments favored a nopunctuation, nonoverlapping model ( g. 11.30). An overlapping code would be subject to two restrictions. First, a change in one base (a mutation) could affect more than one codon and thus affect more than one amino acid. But studies of amino acid sequences almost always showed that only one amino acid was changed, which argued against codon overlap. Second, certain restrictions affected which amino acids occurred next to each other in proteins. For example, the amino acid UUU coded could never be adjacent to the amino acid coded by AAA because one or both (depending on the number of bases overlapped) of the overlap codons UUA and UAA would always insert other amino acids between them. Overlap, then, seemed to be ruled out since every amino acid appears next to every other amino acid in one protein or another. Punctuation between codons was also tentatively ruled out. The messenger RNA in the tobacco necrosis satellite virus has just about enough codons to specify its coat protein with no room left for a punctuating base or bases between each codon.

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where 1 and 2 are the two frequencies that determine the pass-band (or bandwidth) of the lter that is, the frequency range over which the lter passes the input signal and A is a constant that results from the factoring An immediate observation we can make is that if the signal frequency, , is zero, the response of the lter is equal to zero, since at = 0 the impedance of the capacitor, 1/j C, becomes in nite Thus, the capacitor acts as an open circuit, and the output voltage equals zero Further, we note that the lter output in response to an input signal at sinusoidal frequency approaching in nity is again equal to zero This result can be veri ed by considering that as approaches in nity, the impedance of the inductor becomes in nite, that is, an open circuit Thus, the lter cannot pass signals at very high frequencies In an intermediate band of frequencies, the band-pass lter circuit will provide a variable attenuation of the input signal, dependent on the frequency of the excitation This may be veri ed by taking a closer look at

A inserted after third A of normal (GCA repeat)

The diode peak detector is a circuit capable of tracking the sinusoidal peaks without exhibiting the oscillations of the bridge output voltage The peak detector operates by rectifying and ltering the bridge output in a manner similar to that of the circuit of Figure 847 The ideal peak detector circuit is shown in Figure 867, and the response of a practical peak detector is shown in Figure 868 Its operation is based on the recti cation property of the diode, coupled with the ltering effect of the shunt capacitor, which acts as a low-pass lter

~ vout(t)

Frameshift Deletion and insertion combined (return to CAG repeat) * * C G C A G C A A G C A G C A G

Using the fact that X = f/R <<< 1 and q is small, Z becomes Z = R ( sin X ) = R ( sin f R )

005 004 Volts 003 002 001 0 0 01 02 05 06 07 08 t (s) Peak-detected bridge output voltage 03 04 09 1

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