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However, since the Si-based technology is more widespread and much cheaper than the groups III V technology, it would be desirable if e ective Si-based optical materials can be developed An area with some potential promise is Si and SiO2 with nanocrystalline inclusions Silicon layers can be fabricated by molecular beam epitaxy (MBE) which are doped with In and As at levels above their solubility as well as SiO2 layers doped with Ge With MBE it is possible to grow layers ( 1 nm) embedded in undoped Si lms The layers can be grown into a diode structure with optical properties [33] Nanotechnology (nanophotonics and nanoelectronics) is being studied as a way to break through the design restrictions and bottlenecks Nanophotonics is discussed in greater detail in 5; the topic of electronics, speci cally nanoelectronics, is discussed in more detail in 6..

high electroosmotic ow (Section 5.10.1) which places an effective lower limit on the mass of nucleic acid it is possible to separate. While newer commercially-available agaroses are almost capable of matching their impressive resolving power, in practice polyacrylamide gels are normally used to study nucleic acids of smaller mass (e.g. <200 bp). We have seen in Section 5.7 that agarose gels are largely nonrestrictive for proteins. However, compared to most proteins, nucleic acids are extremely long molecules with a large axial ratio (ratio of longitudinal to transverse axes of the molecule). Since, during migration through the gel the nucleic acid is free to rotate through all possible orientations, it migrates as if it has an extremely large molecular mass (Figure 5.35). The same is true for proteins but, because their axial ratios are much smaller than nucleic acids, the apparent mass is much closer to the actual mass. This means that agarose gels, while not restrictive for compact proteins, are restrictive for nucleic acids and should give separation based on differences in charge and mass. As nucleic acids have a fairly uniform charge-distribution, this means that they separate in practice according to differences in mass. Agarose gels are normally used in the concentration range 0.8 1.5% allowing separation of molecules up to 50 kb (although 20 kb is the upper limit for high-resolution separations). Above 20 kb, separation of DNA becomes increasingly independent of molecular mass. This has been explained by two principal models (Figure 5.36). In the biased-reptation model, a DNA molecule larger than the average pore-size of an agarose gel is proposed to display the motion of a crawling snake (i.e. reptation) adopting elongated shapes orientated in the direction of the electric eld. Large DNA molecules will therefore slither through the agarose pores

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DCL commands allow you control access to data. Depending on the database, you may be able to control user privileges at the database, table, or eld level. The following table summarizes the two most common DCL commands. Command

This systematic error due to plotter distortion can be calibrated when the plotter distortion is known (Wyant et al, 1974) When the maximum plotting error is equal to one-half of the resolution spot size, the sensitivity of the CGH test W is given by 4S=P, where P is the number of distortion-free plotter points Using an e-beam recorder with 025-mm resolution over a l0-mm-diameter hologram would enable the measurement of an aspheric wavefront with a maximum wavefront slope of 1000 waves per radius to be tested to a sensitivity of l/10 (assuming a perfect plotter) 12123 Off-Axis CGH Aspheric Compensator To ensure that there is no overlapping of the rst and second orders in the Fourier plane (where the spatial lter is located), the tilt angle of the reference plane wave needs to be greater than three times the maximum wavefront slope of the aberrated wave.

Figure 16.17 (Continued )

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(Note that there are no even orders for a grating with a 50% duty cycle) A photograph of the diffracted orders from the hologram in the Fourier plane is shown in Figure 1248, and a diagram detailing the necessary separations of the orders in the Fourier plane is shown in Figure 1249 The bandwidth of the Nth order is given by 2NS, where S is the maximum wavefront slope in waves per radius of the wavefront to be reconstructed This bandwidth (diameter of the diffracted beam in the Fourier plane) determines the size of the spatial- ltering aperture By moving the spatial ltering aperture, the output of the interferometer can either be the interference of two plane waves or two aspheric waves.

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